Document 0545
 DOCN  M9480545
 TI    The secreted tumor-associated antigen 90K is a potent immune stimulator.
 DT    9410
 AU    Ullrich A; Sures I; D'Egidio M; Jallal B; Powell TJ; Herbst R; Dreps A;
       Azam M; Rubinstein M; Natoli C; et al; Department of Molecular Biology,
       Max-Planck-Institut fur; Biochemie, Martinsried, Federal Republic of
       Germany.
 SO    J Biol Chem. 1994 Jul 15;269(28):18401-7. Unique Identifier : AIDSLINE
       GENBANK/X79089
 AB    Immunization of mice with conditioned media from human breast cancer
       cells yielded the monoclonal antibody SP-2, which recognized an antigen
       of approximately 90-95 kDa. This protein, designated 90K, was found to
       be present in the serum of healthy individuals and at elevated levels in
       the serum of subpopulations of patients with various types of cancer and
       AIDS. Here we report the primary structure of the SP-2 antigen and
       demonstrate its relationship to a family of proteins which carry a
       scavenger receptor cysteine-rich domain. Northern blot analysis of
       normal tissues, primary tumors, and tumor-derived cell lines indicates a
       broad expression spectrum of the 90K gene at widely varying levels.
       Functional characterization reveals stimulatory effects of 90K on host
       defense systems, such as natural killer cell and lymphokine-activated
       killer cell activity, and indicates that its immunostimulatory effects
       may be mediated through the induction of interleukin-2 and possibly
       other cytokines.
 DE    Acquired Immunodeficiency Syndrome/BLOOD/IMMUNOLOGY  *Adjuvants,
       Immunologic  Amino Acid Sequence  Animal  Antigens,
       Neoplasm/*BIOSYNTHESIS/BLOOD/*IMMUNOLOGY  Ascites/METABOLISM  Base
       Sequence  Biological Markers/BLOOD  Biopsy  Blotting, Northern  Breast
       Neoplasms/*METABOLISM/PATHOLOGY  Cloning, Molecular  Cytotoxicity,
       Immunologic  DNA Probes  Female  Gene Expression  Gene Library  Human
       Killer Cells, Natural/IMMUNOLOGY
       Lipoproteins/*BIOSYNTHESIS/BLOOD/IMMUNOLOGY  Mice  Molecular Sequence
       Data  Neoplasm Proteins/*BIOSYNTHESIS/BLOOD/IMMUNOLOGY
       Neoplasms/BLOOD/IMMUNOLOGY/*METABOLISM  Ovarian Neoplasms/METABOLISM
       Plasmids  RNA, Messenger/BIOSYNTHESIS/METABOLISM  Support, Non-U.S.
       Gov't  Transfection  Tumor Cells, Cultured  Tumor Markers,
       Biological/*BLOOD  3T3 Cells  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).